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For spinal structures with naturally limited blood supply (particularly the intervertebral discs, which are largely avascular), this enhanced circulation to surrounding tissues can make a meaningful difference in healing capacity

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To construct the shRNA knockdown vector pLKO.1-GST DAF , we inserted the GST-EGFP-GPI DAF coding region into the pLKO.1 puro plasmid in place of the puromycin resistance gene

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