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All handling, measuring, and experimental evaluation must be conducted exclusively by qualified scientific professionals within a certified and monitored research facility

Conditions associated with subnormal 5-NT levels Lead poisoning Lead exposure can lead to subnormal levels of 5-NT due to direct inhibitory effects on the enzyme in serum 120,121 and red blood cells

Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing

Inject into the abdomen, rotating sites daily

Elevating the head of the bed 6 to 8 inches provides overnight gravitational protection

Finkel 2011