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6A) showed that si-YTHDF1 transfection significantly down-regulated SLC7A5, whereas the subsequent pcDNA 3.1-SLC7A5 successfully restored its level

The clarified cell lysate was either directly submitted to further protein purification steps or flash-frozen in liquid nitrogen after addition of 10% glycerol and stored at 80 C for use in biocatalytic reactions

So, the infections are probably one of those rate-limiting steps

doi: 10.1017/S0954422418000021 40 McBrideJ

Do they both come from natural sources

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