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Although different reagents, such as dithiothreitol (DTT) or 2-mercaptoethanol (2-ME), are dedicated to the reduction of disulfide bonds in the new assay TCEP was applied

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The injection site can be the thigh, abdomen, or upper arm

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The detection sensitivity reaches the nanogram level, making it suitable for quantifying trace active ingredients in capsules and related pharmacokinetic studies

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