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Compound 2 was added to the solution of ( N - tert -butoxycarbonyl)-L-selenocysteine methyl ester in either methanol or phosphate buffer and incubated for 40 min, 24 h, and 120 h under N 2 gas balloon

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Because copper binds tightly to the histidine and lysine residues, the peptide acts as a physiological carrier that delivers copper to tissues and modulates a wide range of regenerative signals

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5 mM was confirmed to be the optimum GSH concentration (Fig