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Comparing germ-free mice to conventionally grown (CONV-R) mice, it has been shown that the former had increased bone mass due to their changed immunological state, which includes lower pro-inflammatory cytokines, decreased CD4+ T cells, and fewer osteoclasts/precursors in the bone marrow (220)
Differences between groups were compared via two-tailed unpaired t tests for two groups, one-way analysis of variance (ANOVA) followed by Dunnetts multiple comparison test for multiple groups, or two-way repeated-measures ANOVA followed by the Bonferroni multiple comparison test for multiple groups and time courses
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These are usually adaptation symptoms rather than long-term side effects and often subside as your body grows accustomed to the GLP-1 hormone
10.1089/ars.2024.0586 43 JungE.JungW
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