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In response to growth signals, mTORC1 activates ATF4, which stimulates the expression of core enzymes in serine synthesis and the folate cycle for de novo purine synthesis required for DNA replication and ribosome biogenesis in rapidly growing and proliferating tumor cells (Denicola et al., 2015

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Patients typically notice visible results within four to six weeks

Conversely, some individuals who initially tolerated high doses find that after substantial weight loss, lower doses provide adequate maintenance with fewer side effects