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The cDNA was synthesized from each RNA sample by reverse transcription using the Revert Aid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, K1622)
After 48 h incubation of confluent monolayers with cARLA, the culture medium was removed and hECs were fixed with a 1:1 mixture of ice cold methanol-acetone solution for 2 min
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Metabolomic analysis further demonstrated clearance of lipotoxic intermediates, decreased pro-inflammatory lipids, reduced collagen-derived metabolites, and elevated anti-inflammatory 15-HETE, confirming metabolic restoration at multiple regulatory levels
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